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PCGF6 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Polycomb group ring finger 6

Gene Aliases

MBLR; Mel18 and Bmi1-like RING finger; Mel18 and Bmi1-like RING finger protein; polycomb group RING finger protein 6; ring finger protein 134; RNF134.

Gene ID

84108

Accession Number

NP_001011663.1

Reactivity

Homo sapiens, Human

Target

Polycomb group RING finger protein 6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PCGF6 ELISA Kit (Human) (OKEH02203) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PCGF6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PCGF6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PCGF6 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

20 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

39 kDa

Specificity

Natural and recombinant Human Polycomb group RING finger protein 6

Protein Length

350

NCBI Gene Symbol

PCGF6

Protein Name

Q9BYE7

Gene Name URL

PCGF6

Nucleotide Accession Number

NM_001011663.1

Frequently Asked Questions

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