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PDE1C ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Phosphodiesterase 1C

Gene Aliases

3',5'-cyclic-AMP phosphodiesterase;3',5'-cyclic-GMP phosphodiesterase; calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C; cam-PDE 1C; DFNA74; hCam-3; Hcam3; Human 3',5' cyclic nucleotide phosphodiesterase (HSPDE1C1A) ; phosphodiesterase 1C, calmodulin-dependent 70kDa.

Gene ID

5137

Accession Number

NP_001177985.1

Reactivity

Homo sapiens, Human

Target

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PDE1C ELISA Kit (Human) (OKEH02197) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDE1C has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDE1C is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDE1C captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%

Sensitivity

0.15 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

81 kDa

Specificity

Natural and recombinant Human Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C

Protein Length

709

NCBI Gene Symbol

PDE1C

Protein Name

Q14123

Gene Name URL

PDE1C

Nucleotide Accession Number

NM_001191056.2

Frequently Asked Questions

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