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ZP3 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Zona pellucida glycoprotein 3

Gene Aliases

OOMD3; sperm receptor; Zona pellucida glycoprotein 3; zona pellucida glycoprotein 3B; zona pellucida glycoprotein ZP3; zona pellucida protein C; zona pellucida sperm-binding protein 3; Zp-3; ZP3A; ZP3A/ZP3B; ZP3B; ZPC.

Gene ID

7784

Accession Number

NP_001103824.1

Reactivity

Homo sapiens, Human

Target

Zona pellucida sperm-binding protein 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ZP3 ELISA Kit (Human) (OKEH02192) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ZP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ZP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ZP3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.625-40ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%

Sensitivity

0.156 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

47 kDa

Specificity

Natural and recombinant Human Zona pellucida sperm-binding protein 3

Protein Length

424

NCBI Gene Symbol

ZP3

Protein Name

P21754

Gene Name URL

ZP3

Nucleotide Accession Number

NM_001110354.1

Frequently Asked Questions

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