HERPUD1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Homocysteine inducible ER protein with ubiquitin like domain 1
Gene Aliases
HERP; homocysteine-inducible endoplasmic reticulum stress-inducible ubiquitin-like domain member 1 protein; homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1; homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein; methyl methanesulfonate (MMF) -inducible fragment protein 1; Mif1; MMS-inducible; SUP.
Gene ID
9709
Accession Number
NP_001010989.1
Reactivity
Homo sapiens, Human
Target
Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HERPUD1 ELISA Kit (Human) (OKEH02170) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HERPUD1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HERPUD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HERPUD1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
Sensitivity
0.1 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
44 kDa
Specificity
Natural and recombinant Human Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein
Protein Length
391
NCBI Gene Symbol
HERPUD1
Protein Name
Q15011
Gene Name URL
HERPUD1
Nucleotide Accession Number
NM_001010989.2
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