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C4B ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Complement C4B (Chido blood group), complement component 4B (Chido blood group), copy 2

Gene Aliases

Basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3; C4B_2; C4B1; C4B12; C4B2; C4B3; C4B5; C4BD; C4F; CH; Chido form of C4; CO4; complement C4-B; complement C4B1a; complement C4-B-like; complement component 4B (Chido blood group) ; CPAMD3.

Gene ID

100293534, 721

Accession Number

NP_001002029.3

Reactivity

Homo sapiens, Human

Target

Complement C4-B

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology C4B ELISA Kit (Human) (OKEH02150) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C4B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C4B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C4B captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%

Sensitivity

0.25 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

193 kDa

Specificity

Natural and recombinant Human Complement C4-B

Protein Length

1744

NCBI Gene Symbol

C4B, C4B_2

Protein Name

P0C0L5

Gene Name URL

C4B, C4B_2

Nucleotide Accession Number

NM_001002029.3

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