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TBXAS1 ELISA Kit (Human)

Selected Variant

OKEH02147-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Thromboxane A synthase 1

Gene Aliases

BDPLT14; CYP5; CYP5A1; cytochrome P450 5A1; cytochrome P450, family 5, subfamily A, polypeptide 1; GHOSAL; hydroperoxy icosatetraenoate dehydratase; platelet, cytochrome P450, subfamily V; THAS; thromboxane A synthase 1 (platelet) ; thromboxane-A synthase; TS; TXA synthase; TXAS; TXS.

Gene ID

6916

Accession Number

NP_001052.2

Reactivity

Homo sapiens, Human

Target

Thromboxane-A synthase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TBXAS1 ELISA Kit (Human) (OKEH02147) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TBXAS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TBXAS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TBXAS1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 82%

Sensitivity

0.067 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

61 kDa

Specificity

Natural and recombinant Human Thromboxane-A synthase

Protein Length

533

NCBI Gene Symbol

TBXAS1

Protein Name

P24557

Gene Name URL

TBXAS1

Nucleotide Accession Number

NM_001061.4

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