REG3A ELISA Kit (Human)

Hepatocarcinoma-intestine-pancreas; hepatointestinal pancreatic protein; HIP; HIP/PAP; human proislet peptide; INGAP; pancreatic beta cell growth factor; pancreatitis-associated protein 1; PAP; PAP homologous protein; PAP1; PAP-H; PBCGF; proliferation-inducing protein 34; proliferation-inducing protein 42; reg III-alpha; REG3; REG-3-alpha; regenerating islet-derived 3 alpha; regenerating islet-derived protein 3-alpha; regenerating islet-derived protein III-alpha; REG-III.

Aviva Systems Biology REG3A ELISA Kit (Human) (OKEH02145) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for REG3A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for REG3A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample REG3A captured in well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol