IFI6 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Interferon alpha inducible protein 6
Gene Aliases
6-16; FAM14C; G1P3; IFI616; IFI-6-16; interferon alpha-inducible protein 6; interferon, alpha-inducible protein clone IFI-6-16; interferon-induced protein 6-16.
Gene ID
2537
Accession Number
NP_002029.3
Reactivity
Homo sapiens, Human
Target
Interferon alpha-inducible protein 6
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology IFI6 ELISA Kit (Human) (OKEH02144) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IFI6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IFI6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IFI6 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
Sensitivity
7.9 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
13 kDa
Specificity
Natural and recombinant Human Interferon alpha-inducible protein 6
Protein Length
130
NCBI Gene Symbol
IFI6
Protein Name
P09912
Gene Name URL
IFI6
Nucleotide Accession Number
NM_002038.3
Frequently Asked Questions
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