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BCL6B ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

BCL6B transcription repressor

Gene Aliases

B cell CLL/lymphoma 6B; BAZF; B-cell CLL/lymphoma 6 member B protein; B-cell CLL/lymphoma 6, member B (zinc finger protein) ; bcl6-associated zinc finger protein; ZBTB28; zinc finger protein 62; ZNF62.

Gene ID

255877

Accession Number

NP_862827.1

Reactivity

Homo sapiens, Human

Target

B-cell CLL/lymphoma 6 member B protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology BCL6B ELISA Kit (Human) (OKEH02140) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL6B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCL6B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCL6B captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

0.34 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

52 kDa

Specificity

Natural and recombinant Human B-cell CLL/lymphoma 6 member B protein

Protein Length

479

NCBI Gene Symbol

BCL6B

Protein Name

Q8N143

Gene Name URL

BCL6B

Nucleotide Accession Number

NM_181844.3

Frequently Asked Questions

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