SLC7A11 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Solute carrier family 7 member 11
Gene Aliases
Amino acid transport system xc-; calcium channel blocker resistance protein CCBR1; CCBR1; cystine/glutamate transporter; solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11; Solute carrier family 7 member 11; solute carrier family 7, (cationic amino acid transporter, y+ system) member 11; xCT.
Gene ID
23657
Accession Number
NP_055146.1
Reactivity
Homo sapiens, Human
Target
Cystine/glutamate transporter
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SLC7A11 ELISA Kit (Human) (OKEH02129) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC7A11 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC7A11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC7A11 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
55 kDa
Specificity
Natural and recombinant Human Cystine/glutamate transporter
Protein Length
501
NCBI Gene Symbol
SLC7A11
Protein Name
Q9UPY5
Gene Name URL
SLC7A11
Nucleotide Accession Number
NM_014331.3
Frequently Asked Questions
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