IQGAP1 ELISA Kit (Human)
Selected Variant
OKEH02125-96W
Size: 96 Well
Product Specifications
CAS Number
7732-18-5
Gene Name
IQ motif containing GTPase activating protein 1
Gene Aliases
HUMORFA01; p195; ras GTPase-activating-like protein IQGAP1; RasGAP-like with IQ motifs; SAR1.
Gene ID
8826
Accession Number
NP_003861.1
Reactivity
Homo sapiens, Human
Target
Ras GTPase-activating-like protein IQGAP1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology IQGAP1 ELISA Kit (Human) (OKEH02125) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IQGAP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IQGAP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IQGAP1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 114%
Sensitivity
0.045 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
189 kDa
Specificity
Natural and recombinant Human Ras GTPase-activating-like protein IQGAP1
Protein Length
1657
NCBI Gene Symbol
IQGAP1
Protein Name
P46940
Gene Name URL
IQGAP1
Nucleotide Accession Number
NM_003870.3
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