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AKR1B10 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Aldo-keto reductase family 1 member B10

Gene Aliases

AKR1B11; AKR1B12; aldo-keto reductase family 1 member B10; aldo-keto reductase family 1, member B10 (aldose reductase) ; aldo-keto reductase family 1, member B11 (aldose reductase-like) ; Aldose reductase-like; aldose reductase-like 1; aldose reductase-like peptide; aldose reductase-related protein; ALDRLn; ARL1; ARL-1; ARP; hARP; HIS; HSI; SI reductase; small intestine reductase.

Gene ID

57016

Accession Number

NP_064695.3

Reactivity

Homo sapiens, Human

Target

Aldo-keto reductase family 1 member B10

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology AKR1B10 ELISA Kit (Human) (OKEH02121) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for AKR1B10 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AKR1B10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AKR1B10 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%

Sensitivity

0.23 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

36 kDa

Specificity

Natural and recombinant Human Aldo-keto reductase family 1 member B10

Protein Length

316

NCBI Gene Symbol

AKR1B10

Protein Name

O60218

Gene Name URL

AKR1B10

Nucleotide Accession Number

NM_020299.4

Frequently Asked Questions

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