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BIRC2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Baculoviral IAP repeat containing 2

Gene Aliases

API1; apoptosis inhibitor 1; baculoviral IAP repeat-containing protein 2; cellular inhibitor of apoptosis 1; cIAP1; c-IAP1; Hiap-2; HIAP2; IAP homolog B; IAP-2; inhibitor of apoptosis protein 2; MIHB; NFR2-TRAF signalling complex protein; RING finger protein 48; RING-type E3 ubiquitin transferase BIRC2; RNF48; TNFR2-TRAF-signaling complex protein 2.

Gene ID

329

Accession Number

NP_001157.1

Reactivity

Homo sapiens, Human

Target

Baculoviral IAP repeat-containing protein 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology BIRC2 ELISA Kit (Human) (OKEH02117) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BIRC2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BIRC2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BIRC2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%

Sensitivity

34 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

70 kDa

Specificity

Natural and recombinant Human Baculoviral IAP repeat-containing protein 2

Protein Length

618

NCBI Gene Symbol

BIRC2

Protein Name

Q13490

Gene Name URL

BIRC2

Nucleotide Accession Number

NM_001166.4

Frequently Asked Questions

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