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AKAP17A ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

A-kinase anchoring protein 17A

Gene Aliases

721P; A kinase (PRKA) anchor protein 17A; AKAP-17A; A-kinase anchor protein 17A; B-lymphocyte antigen; B-lymphocyte surface antigen; CCDC133; CXYorf3; DXYS155E; PRKA17A; protein kinase A-anchoring protein 17A; Protein XE7; pseudoautosomal gene XE7; SFRS17A; splicing factor, arginine/serine-rich 17A; XE7; XE7Y.

Gene ID

8227

Accession Number

NP_005079.2

Reactivity

Homo sapiens, Human

Target

A-kinase anchor protein 17A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology AKAP17A ELISA Kit (Human) (OKEH02112) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AKAP17A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AKAP17A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AKAP17A captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%

Sensitivity

0.045 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

81 kDa

Specificity

Natural and recombinant Human A-kinase anchor protein 17A

Protein Length

695

NCBI Gene Symbol

AKAP17A

Protein Name

Q02040

Gene Name URL

AKAP17A

Nucleotide Accession Number

NM_005088.2

Frequently Asked Questions

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