AKAP17A ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
A-kinase anchoring protein 17A
Gene Aliases
721P; A kinase (PRKA) anchor protein 17A; AKAP-17A; A-kinase anchor protein 17A; B-lymphocyte antigen; B-lymphocyte surface antigen; CCDC133; CXYorf3; DXYS155E; PRKA17A; protein kinase A-anchoring protein 17A; Protein XE7; pseudoautosomal gene XE7; SFRS17A; splicing factor, arginine/serine-rich 17A; XE7; XE7Y.
Gene ID
8227
Accession Number
NP_005079.2
Reactivity
Homo sapiens, Human
Target
A-kinase anchor protein 17A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology AKAP17A ELISA Kit (Human) (OKEH02112) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AKAP17A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AKAP17A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AKAP17A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
Sensitivity
0.045 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
81 kDa
Specificity
Natural and recombinant Human A-kinase anchor protein 17A
Protein Length
695
NCBI Gene Symbol
AKAP17A
Protein Name
Q02040
Gene Name URL
AKAP17A
Nucleotide Accession Number
NM_005088.2
Frequently Asked Questions
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