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NR1I2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Nuclear receptor subfamily 1 group I member 2

Gene Aliases

BXR; nuclear receptor subfamily 1 group I member 2; ONR1; orphan nuclear receptor PAR1; orphan nuclear receptor PXR; PAR; PAR1; PAR2; PARq; pregnane X nuclear receptor variant 2; pregnane X receptor; PRR; PXR; SAR; steroid and xenobiotic receptor; SXR.

Gene ID

8856

Accession Number

NP_003880.3

Reactivity

Homo sapiens, Human

Target

Nuclear receptor subfamily 1 group I member 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NR1I2 ELISA Kit (Human) (OKEH02107) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NR1I2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NR1I2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NR1I2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%

Sensitivity

0.045 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

50 kDa

Specificity

Natural and recombinant Human Nuclear receptor subfamily 1 group I member 2

Protein Length

434

NCBI Gene Symbol

NR1I2

Protein Name

O75469

Gene Name URL

NR1I2

Nucleotide Accession Number

NM_003889.3

Frequently Asked Questions

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