SIGLEC10 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Sialic acid binding Ig like lectin 10
Gene Aliases
PRO940; sialic acid binding Ig-like lectin 10 Ig-like lectin 7; sialic acid-binding Ig-like lectin 10; SIGLEC-10; siglec-like gene 2; siglec-like protein 2; SLG2.
Gene ID
89790
Accession Number
NP_001164627.1
Reactivity
Homo sapiens, Human
Target
Sialic acid-binding Ig-like lectin 10
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SIGLEC10 ELISA Kit (Human) (OKEH02104) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SIGLEC10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SIGLEC10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SIGLEC10 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 1%
Sensitivity
0.098 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
77 kDa
Specificity
Natural and recombinant Human Sialic acid-binding Ig-like lectin 10
Protein Length
697
NCBI Gene Symbol
SIGLEC10
Protein Name
Q96LC7
Gene Name URL
SIGLEC10
Nucleotide Accession Number
NM_001171156.1
Frequently Asked Questions
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