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PTPRQ ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Protein tyrosine phosphatase, receptor type Q

Gene Aliases

Receptor-type tyrosine-protein phosphatase Q.

Gene ID

374462

Accession Number

NP_001138498.1

Reactivity

Homo sapiens, Human

Target

Phosphatidylinositol phosphatase PTPRQ

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology PTPRQ ELISA Kit (Human) (OKEH02080) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human PTPRQ antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PTPRQ and incubated. The PTPRQ found in the sample or standards competes with the biotinylated PTPRQ for limited binding sites on the immobilized anti-Human PTPRQ antibody. Excess unbound biotinylated PTPRQ and sample or standard PTPRQ is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PTPRQ captured in the well and inversely proportional to the amount of PTPRQ which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%

Sensitivity

0.23 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

257 kDa

Specificity

Natural and recombinant Human Phosphatidylinositol phosphatase PTPRQ

Protein Length

2299

NCBI Gene Symbol

PTPRQ

Protein Name

Q9UMZ3

Gene Name URL

PTPRQ

Nucleotide Accession Number

NM_001145026.1

Frequently Asked Questions

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