What is the catalog number for CYP27B1 ELISA Kit (Human)?

The catalog number for CYP27B1 ELISA Kit (Human) is OKEH02077-96W.

What sizes are available for CYP27B1 ELISA Kit (Human)?

CYP27B1 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is CYP27B1 ELISA Kit (Human) in stock?

CYP27B1 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

CYP27B1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Cytochrome P450 family 27 subfamily B member 1

Gene Aliases

1alpha (OH) ase;25 hydroxyvitamin D3-1-alpha hydroxylase;25-hydroxyvitamin D (3) 1-alpha-hydroxylase;25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial;25-OHD-1 alpha-hydroxylase; calcidiol 1-monooxygenase; CP2B; CYP1; CYP1alpha; CYP27B; cytochrome p450 27B1; cytochrome P450 subfamily XXVIIB polypeptide 1; cytochrome P450, family 27, subfamily B, polypeptide 1; cytochrome P450C1 alpha; cytochrome P450VD1-alpha; P450c1; PDDR; VD3 1A hydroxylase; VDD1; VDDR; VDDRI; VDR.

Gene ID

1594

Accession Number

NP_000776.1

Reactivity

Homo sapiens, Human

Target

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CYP27B1 ELISA Kit (Human) (OKEH02077) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP27B1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP27B1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYP27B1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol