CYP1A1 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Cytochrome P450, family 1, subfamily a, polypeptide 1
Gene Aliases
AHH; AHRR; aryl hydrocarbon hydroxylase; CP11; CYP1; Cyp45c; Cypc45c; CYPIA1; cytochrome P1-450; cytochrome P450 1A1; cytochrome P450 form 6; cytochrome P450 subfamily I (aromatic compound-inducible) member A1 (C6 form c) (C6 form c) ; cytochrome P450, 1a1; cytochrome P450, subfamily I (aromatic compound-inducible), member A1 (C6, form c) (C6, form c) ; cytochrome P450-C; cytochrome P450MT2; cytochrome P450-P1; dioxin-inducible; flavoprotein-linked monooxygenase; hydroperoxy icosatetraenoate dehydratase; microsomal monooxygenase; P1-450; P450-C; P450DX; P450form6; P-450MC; P450MT2; xenobiotic monooxygenase.
Gene ID
24296
Accession Number
NP_036672.2
Reactivity
Rat, Rattus norvegicus
Target
Cytochrome P450 1A1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CYP1A1 ELISA Kit (Rat) (OKEH02059) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP1A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP1A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYP1A1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
59 kDa
Specificity
Natural and recombinant Rat Cytochrome P450 1A1
Protein Length
524
NCBI Gene Symbol
Cyp1a1
Protein Name
P00185
Gene Name URL
Cyp1a1
Nucleotide Accession Number
NM_012540.2
Frequently Asked Questions
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