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MT-CO3 ELISA Kit (Pig)

Product Specifications

CAS Number

7732-18-5

Gene Name

Cytochrome c oxidase subunit III

Gene Aliases

Cytochrome c oxidase polypeptide III; cytochrome c oxidase subunit III; KEF22_p07.

Gene ID

808507

Accession Number

NP_008640.1

Reactivity

Porcine, Sus scrofa

Target

Cytochrome c oxidase subunit 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MT-CO3 ELISA Kit (Pig) (OKEH02053) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MT-CO3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MT-CO3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MT-CO3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%

Sensitivity

0.051 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

29 kDa

Specificity

Natural and recombinant Pig Cytochrome c oxidase subunit 3

Protein Length

261

NCBI Gene Symbol

COX3

Protein Name

Q35916

Gene Name URL

COX3

Nucleotide Accession Number

NC_000845.1

Frequently Asked Questions

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