KRT6A ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Keratin 6A
Gene Aliases
CK6A; CK6C; CK-6C; CK6D; CK-6E; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; Cytokeratin-6A; Cytokeratin-6D; K6A; K6C; K6D; keratin 6A, , type II; keratin 6A, type II; keratin, epidermal type II, K6A; keratin, type II cytoskeletal 6A; Keratin-6A; KRT6C; KRT6D; PC3; type-II keratin Kb6.
Gene ID
3853
Accession Number
NP_005545.1
Reactivity
Homo sapiens, Human
Target
Keratin, type II cytoskeletal 6A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology KRT6A ELISA Kit (Human) (OKEH02048) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KRT6A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KRT6A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KRT6A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
15 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
60 kDa
Specificity
Natural and recombinant Human Keratin, type II cytoskeletal 6A
Protein Length
564
NCBI Gene Symbol
KRT6A
Protein Name
P02538
Gene Name URL
KRT6A
Nucleotide Accession Number
NM_005554.3
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