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RNASE7 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Ribonuclease A family member 7

Gene Aliases

RAE1; ribonuclease 7; ribonuclease, RNase A family, 7; RNase 7; SAP-2; skin-derived antimicrobial protein 2.

Gene ID

84659

Accession Number

NP_115961.2

Reactivity

Homo sapiens, Human

Target

Ribonuclease 7

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology RNASE7 ELISA Kit (Human) (OKEH02047) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RNASE7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RNASE7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RNASE7 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Sensitivity

23 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

17 kDa

Specificity

Natural and recombinant Human Ribonuclease 7

Protein Length

156

NCBI Gene Symbol

RNASE7

Protein Name

Q9H1E1

Gene Name URL

RNASE7

Nucleotide Accession Number

NM_032572.3

Frequently Asked Questions

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