FCGR3B ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Fc fragment of IgG receptor IIIb
Gene Aliases
CD16; CD16A; CD16b; Fc fragment of IgG, low affinity IIIb, receptor (CD16b) ; Fc gamma receptor IIIb; Fc gamma receptor III-B; FCG3; Fc-gamma receptor IIIb (CD 16) ; fc-gamma RIIIb; fc-gamma RIII-beta; FCGR3; FCGR3A; FCR-10; FCRIII; FCRIIIb; igG Fc receptor III-1; low affinity immunoglobulin gamma Fc region receptor III-B.
Gene ID
2215
Accession Number
NP_000561.3
Reactivity
Homo sapiens, Human
Target
Low affinity immunoglobulin gamma Fc region receptor III-B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FCGR3B ELISA Kit (Human) (OKEH02039) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FCGR3B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FCGR3B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FCGR3B captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
<= 4.9%
Sensitivity
0.11 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
26 kDa
Specificity
Natural and recombinant Human Low affinity immunoglobulin gamma Fc region receptor III-B
Protein Length
233
NCBI Gene Symbol
FCGR3B
Protein Name
O75015
Gene Name URL
FCGR3B
Nucleotide Accession Number
NM_000570.4
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