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GPX3 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Glutathione peroxidase 3

Gene Aliases

Extracellular glutathione peroxidase; glutathione peroxidase 3; glutathione peroxidase 3 (plasma) ; GPx-3; GPx-P; GSHPx-3; GSHPx-P; plasma glutathione peroxidase; selenoprotein GPX3.

Gene ID

2878

Accession Number

NP_002075.2

Reactivity

Homo sapiens, Human

Target

Glutathione peroxidase 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology GPX3 ELISA Kit (Human) (OKEH02034) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human GPX3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GPX3 and incubated. The GPX3 found in the sample or standards competes with the biotinylated GPX3 for limited binding sites on the immobilized anti-Human GPX3 antibody. Excess unbound biotinylated GPX3 and sample or standard GPX3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GPX3 captured in the well and inversely proportional to the amount of GPX3 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

1.56-100ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%

Sensitivity

0.23 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

257 kDa

Specificity

Natural and recombinant Human Glutathione peroxidase 3

Protein Length

229

NCBI Gene Symbol

GPX3

Protein Name

P22352

Gene Name URL

GPX3

Nucleotide Accession Number

NM_002084.3

Frequently Asked Questions

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