TGFBR2 ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Transforming growth factor, beta receptor II
Gene Aliases
1110020H15Rik; AU042018; DNIIR; RIIDN; Tbet; Tbeta; TbetaRII; TbetaR-II; TBR; TBR-II; TGF-beta receptor II; TGF-beta receptor type II; TGF-beta receptor type-2; TGF-beta type II receptor; TGFR-2; transforming growth factor-beta receptor type II.
Gene ID
21813
Accession Number
NP_033397.3
Reactivity
Mouse, Mus musculus
Target
TGF-beta receptor type-2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TGFBR2 ELISA Kit (Mouse) (OKEH02032) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TGFBR2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TGFBR2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TGFBR2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
7.8 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
67 kDa
Notes
Formerly GWB-V17PMP
Specificity
Natural and recombinant Mouse TGF-beta receptor type-2
Protein Length
592
NCBI Gene Symbol
Tgfbr2
Protein Name
Q62312
Gene Name URL
Tgfbr2
Nucleotide Accession Number
NM_009371.3
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