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SKP2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

S-phase kinase associated protein 2

Gene Aliases

CDK2/cyclin A-associated protein p45; Cyclin-A/CDK2-associated protein p45; FBL1; F-box protein Skp2; F-box/LRR-repeat protein 1; FBXL1; FLB1; p45; p45skp2; S-phase kinase-associated protein 2; S-phase kinase-associated protein 2, E3 ubiquitin protein ligase.

Gene ID

6502

Accession Number

NP_001230049.1

Reactivity

Homo sapiens, Human

Target

S-phase kinase-associated protein 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SKP2 ELISA Kit (Human) (OKEH02026) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SKP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SKP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SKP2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%

Sensitivity

0.089 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

48 kDa

Specificity

Natural and recombinant Human S-phase kinase-associated protein 2

Protein Length

424

NCBI Gene Symbol

SKP2

Protein Name

Q13309

Gene Name URL

SKP2

Nucleotide Accession Number

NM_001243120.1

Frequently Asked Questions

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