IKBKG ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Inhibitor of nuclear factor kappa B kinase regulatory subunit gamma
Gene Aliases
14.7K (adenovirus E3 protein) interacting protein 3; AMCBX1; EDAID1; FIP3; FIP-3; Fip3p; I-kappa-B kinase subunit gamma; ikB kinase subunit gamma; ikB kinase-associated protein 1; IKKAP1; IKKG; IKK-gamma; IMD33; incontinentia pigmenti; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma; inhibitor of nuclear factor kappa B kinase subunit gamma; Inhibitor of nuclear factor kappa-B kinase subunit gamma; IP; IP1; IP2; IPD2; NEMO; NF-kappa-B essential modifier; NF-kappa-B essential modulator; ZC2HC9.
Gene ID
8517
Accession Number
NP_001093326.2
Reactivity
Homo sapiens, Human
Target
NF-kappa-B essential modulator
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology IKBKG ELISA Kit (Human) (OKEH02025) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IKBKG has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IKBKG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IKBKG captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
0.34 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
48 kDa
Specificity
Natural and recombinant Human NF-kappa-B essential modulator
Protein Length
419
NCBI Gene Symbol
IKBKG
Protein Name
Q9Y6K9
Gene Name URL
IKBKG
Nucleotide Accession Number
NM_001099856.3
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