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DPEP1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Dipeptidase 1

Gene Aliases

Beta-lactamase; dehydropeptidase-I; dipeptidase 1; dipeptidase 1 (renal) ; hRDP; MBD1; MDP; microsomal dipeptidase; RDP; renal dipeptidase; testicular tissue protein Li 57.

Gene ID

1800

Accession Number

NP_001121613.1

Reactivity

Homo sapiens, Human

Target

Dipeptidase 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology DPEP1 ELISA Kit (Human) (OKEH01995) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human DPEP1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated DPEP1 and incubated. The DPEP1 found in the sample or standards competes with the biotinylated DPEP1 for limited binding sites on the immobilized anti-human DPEP1 antibody. Excess unbound biotinylated DPEP1 and sample or standard DPEP1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated DPEP1 captured in the well and inversely proportional to the amount of DPEP1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

45 kDa

Specificity

Natural and recombinant Human Dipeptidase 1

Protein Length

411

NCBI Gene Symbol

DPEP1

Protein Name

P16444

Gene Name URL

DPEP1

Nucleotide Accession Number

NM_001128141.2

Frequently Asked Questions

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