MANF ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Mesencephalic astrocyte-derived neurotrophic factor

Gene Aliases

Arginine-rich protein; arginine-rich, mutated in early stage tumors; Armet; mesencephalic astrocyte-derived neurotrophic factor; Protein ARMET.

Gene ID

315989

Accession Number

XP_006243837.1

Reactivity

Rat, Rattus norvegicus

Target

Mesencephalic astrocyte-derived neurotrophic factor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MANF ELISA Kit (Rat) (OKEH01986) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MANF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MANF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MANF captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%

Sensitivity

15.65 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

20 kDa

Specificity

Natural and recombinant Rat Mesencephalic astrocyte-derived neurotrophic factor

Protein Length

179

NCBI Gene Symbol

Manf

Protein Name

P0C5H9

Gene Name URL

Manf

Nucleotide Accession Number

XM_006243775.1

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