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PPP1CA ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Protein phosphatase 1 catalytic subunit alpha

Gene Aliases

PP1A; PP-1A; PP1alpha; PPP1A; protein phosphatase 1, catalytic subunit, alpha isozyme; serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit; serine/threonine-protein phosphatase PP1-alpha catalytic subunit; testicular tissue protein Li 155.

Gene ID

5499

Accession Number

NP_001008709.1

Reactivity

Homo sapiens, Human

Target

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PPP1CA ELISA Kit (Human) (OKEH01979) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PPP1CA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PPP1CA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PPP1CA captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%

Sensitivity

0.055 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

37 kDa

Specificity

Natural and recombinant Human Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Protein Length

330

NCBI Gene Symbol

PPP1CA

Protein Name

P62136

Gene Name URL

PPP1CA

Nucleotide Accession Number

NM_001008709.1

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