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HMMR ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Hyaluronan mediated motility receptor

Gene Aliases

CD168; hyaluronan mediated motility receptor; IHABP; intracellular hyaluronic acid-binding protein; receptor for hyaluronan-mediated motility; RHAMM.

Gene ID

3161

Accession Number

NP_001136028.1

Reactivity

Homo sapiens, Human

Target

Hyaluronan mediated motility receptor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HMMR ELISA Kit (Human) (OKEH01971) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HMMR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HMMR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HMMR captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

10 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

84 kDa

Specificity

Natural and recombinant Human Hyaluronan mediated motility receptor

Protein Length

724

NCBI Gene Symbol

HMMR

Protein Name

O75330

Gene Name URL

HMMR

Nucleotide Accession Number

NM_001142556.1

Frequently Asked Questions

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