CCT8L1P ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Chaperonin containing TCP1 subunit 8 like 1, pseudogene
Gene Aliases
CCT8L1
Gene ID
155100
Reactivity
Homo sapiens, Human
Target
Putative T-complex protein 1 subunit theta-like 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology CCT8L1P ELISA Kit (Human) (OKEH01970) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human CCT8L1P antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CCT8L1P and incubated. The CCT8L1P found in the sample or standards competes with the biotinylated CCT8L1P for limited binding sites on the immobilized anti-human CCT8L1P antibody. Excess unbound biotinylated CCT8L1P and sample or standard CCT8L1P is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CCT8L1P captured in the well and inversely proportional to the amount of CCT8L1P which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%
Sensitivity
38 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
59 kDa
Specificity
Natural and recombinant Human Putative T-complex protein 1 subunit theta-like 1
Protein Length
557
NCBI Gene Symbol
CCT8L1P
Protein Name
A6NM43
Gene Name URL
CCT8L1P
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog