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CCT8L1P ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Chaperonin containing TCP1 subunit 8 like 1, pseudogene

Gene Aliases

CCT8L1

Gene ID

155100

Reactivity

Homo sapiens, Human

Target

Putative T-complex protein 1 subunit theta-like 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology CCT8L1P ELISA Kit (Human) (OKEH01970) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human CCT8L1P antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CCT8L1P and incubated. The CCT8L1P found in the sample or standards competes with the biotinylated CCT8L1P for limited binding sites on the immobilized anti-human CCT8L1P antibody. Excess unbound biotinylated CCT8L1P and sample or standard CCT8L1P is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CCT8L1P captured in the well and inversely proportional to the amount of CCT8L1P which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%

Sensitivity

38 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

59 kDa

Specificity

Natural and recombinant Human Putative T-complex protein 1 subunit theta-like 1

Protein Length

557

NCBI Gene Symbol

CCT8L1P

Protein Name

A6NM43

Gene Name URL

CCT8L1P

Frequently Asked Questions

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