IGIP ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

IgA inducing protein

Gene Aliases

C5orf53; IgA-inducing protein homolog; IgA-inducing protein homolog (Bos taurus) .

Gene ID

492311

Accession Number

NP_001007190.1

Reactivity

Homo sapiens, Human

Target

IgA-inducing protein homolog

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology IGIP ELISA Kit (Human) (OKEH01967) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human IGIP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated IGIP and incubated. The IGIP found in the sample or standards competes with the biotinylated IGIP for limited binding sites on the immobilized anti-human IGIP antibody. Excess unbound biotinylated IGIP and sample or standard IGIP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated IGIP captured in the well and inversely proportional to the amount of IGIP which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol