IGIP ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
IgA inducing protein
Gene Aliases
C5orf53; IgA-inducing protein homolog; IgA-inducing protein homolog (Bos taurus) .
Gene ID
492311
Accession Number
NP_001007190.1
Reactivity
Homo sapiens, Human
Target
IgA-inducing protein homolog
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology IGIP ELISA Kit (Human) (OKEH01967) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human IGIP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated IGIP and incubated. The IGIP found in the sample or standards competes with the biotinylated IGIP for limited binding sites on the immobilized anti-human IGIP antibody. Excess unbound biotinylated IGIP and sample or standard IGIP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated IGIP captured in the well and inversely proportional to the amount of IGIP which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.1 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
5 kDa
Specificity
Natural and recombinant Human IgA-inducing protein homolog
Protein Length
53
NCBI Gene Symbol
IGIP
Protein Name
A6NJ69
Gene Name URL
IGIP
Nucleotide Accession Number
NM_001007189.1
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