GYP1 ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Glycophorin A
Gene Aliases
AI853584; CD235; CD235a; G; glycophorin-A; GPA.
Gene ID
14934
Accession Number
NP_034499.3
Reactivity
Mouse, Mus musculus
Target
Glycophorin-A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology GYP1 ELISA Kit (Mouse) (OKEH01955) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse GYP1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GYP1 and incubated. The GYP1 found in the sample or standards competes with the biotinylated GYP1 for limited binding sites on the immobilized anti-mouse GYP1 antibody. Excess unbound biotinylated GYP1 and sample or standard GYP1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GYP1 captured in the well and inversely proportional to the amount of GYP1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.312 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
17 kDa
Specificity
Natural and recombinant Mouse Glycophorin-A
Protein Length
168
NCBI Gene Symbol
Gypa
Protein Name
P14220
Gene Name URL
Gypa
Nucleotide Accession Number
NM_010369.3
Frequently Asked Questions
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