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GYP1 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Glycophorin A

Gene Aliases

AI853584; CD235; CD235a; G; glycophorin-A; GPA.

Gene ID

14934

Accession Number

NP_034499.3

Reactivity

Mouse, Mus musculus

Target

Glycophorin-A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology GYP1 ELISA Kit (Mouse) (OKEH01955) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse GYP1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GYP1 and incubated. The GYP1 found in the sample or standards competes with the biotinylated GYP1 for limited binding sites on the immobilized anti-mouse GYP1 antibody. Excess unbound biotinylated GYP1 and sample or standard GYP1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GYP1 captured in the well and inversely proportional to the amount of GYP1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.625-40ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%

Sensitivity

0.312 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

17 kDa

Specificity

Natural and recombinant Mouse Glycophorin-A

Protein Length

168

NCBI Gene Symbol

Gypa

Protein Name

P14220

Gene Name URL

Gypa

Nucleotide Accession Number

NM_010369.3

Frequently Asked Questions

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