S100A10 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
S100 calcium binding protein A10
Gene Aliases
42C; annexin II ligand, calpactin I, light polypeptide; annexin II tetramer (AIIt) p11 subunit; ANX2L; ANX2LG; Ca[1]; CAL1L; calpactin I light chain; calpactin-1 light chain; cellular ligand of annexin II; CLP11; GP11; p10; p10 protein; P11; protein S100-A10; S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11) ) ; S100 calcium-binding protein A10.
Gene ID
6281
Accession Number
NP_002957.1
Reactivity
Homo sapiens, Human
Target
Protein S100-A10
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology S100A10 ELISA Kit (Human) (OKEH01946) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100A10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S100A10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S100A10 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
Sensitivity
0.098 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
11 kDa
Specificity
Natural and recombinant Human Protein S100-A10
Protein Length
97
NCBI Gene Symbol
S100A10
Protein Name
P60903
Gene Name URL
S100A10
Nucleotide Accession Number
NM_002966.2
Frequently Asked Questions
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