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CD63 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

CD63 molecule

Gene Aliases

CD63 antigen; CD63 antigen (melanoma 1 antigen) ; granulophysin; LAMP-3; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; ME491; melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; ocular melanoma-associated antigen; OMA81H; tetraspanin-30; tspan-30; TSPAN30.

Gene ID

967

Accession Number

NP_001244318.1

Reactivity

Homo sapiens, Human

Target

CD63 antigen

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CD63 ELISA Kit (Human) (OKEH01943) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD63 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD63 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD63 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

31.2-2000pg/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%

Sensitivity

19.5 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

26 kDa

Notes

Formerly GWB-TTD47C

Specificity

Natural and recombinant Human CD63 antigen

Protein Length

238

NCBI Gene Symbol

CD63

Protein Name

P08962

Gene Name URL

CD63

Nucleotide Accession Number

NM_001257389.1

Frequently Asked Questions

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