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IRAK3 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Interleukin 1 receptor associated kinase 3

Gene Aliases

ASRT5; IL-1 receptor-associated kinase M; inactive IL-1 receptor-associated kinase 3; interleukin-1 receptor-associated kinase 3; IRAKM.

Gene ID

11213

Accession Number

NP_001135995.1

Reactivity

Homo sapiens, Human

Target

Interleukin-1 receptor-associated kinase 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology IRAK3 ELISA Kit (Human) (OKEH01937) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IRAK3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IRAK3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IRAK3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Sensitivity

20 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

68 kDa

Specificity

Natural and recombinant Human Interleukin-1 receptor-associated kinase 3

Protein Length

596

NCBI Gene Symbol

IRAK3

Protein Name

Q9Y616

Gene Name URL

IRAK3

Nucleotide Accession Number

NM_001142523.1

Frequently Asked Questions

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