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IL2RG ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Interleukin 2 receptor subunit gamma

Gene Aliases

CD132; CD132 antigen; CIDX; common cytokine receptor gamma chain; cytokine receptor common subunit gamma; gammaC; IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; nonfunctional common cytokine receptor gamma chain; P64; SCIDX; SCIDX1.

Gene ID

3561

Accession Number

NP_000197.1

Reactivity

Homo sapiens, Human

Target

Cytokine receptor common subunit gamma

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology IL2RG ELISA Kit (Human) (OKEH01936) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL2RG has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL2RG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL2RG captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

0.098 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

42 kDa

Specificity

Natural and recombinant Human Cytokine receptor common subunit gamma

Protein Length

369

NCBI Gene Symbol

IL2RG

Protein Name

P31785

Gene Name URL

IL2RG

Nucleotide Accession Number

NM_000206.2

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