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JAK2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Janus kinase 2

Gene Aliases

JAK-2; Janus kinase 2; Janus kinase 2 (a protein tyrosine kinase) ; JTK10; tyrosine-protein kinase JAK2.

Gene ID

3717

Accession Number

NP_004963.1

Reactivity

Homo sapiens, Human

Target

Tyrosine-protein kinase JAK2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology JAK2 ELISA Kit (Human) (OKEH01917) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for JAK2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for JAK2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample JAK2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 113%

Sensitivity

0.076 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

131 kDa

Specificity

Natural and recombinant Human Tyrosine-protein kinase JAK2

Protein Length

1132

NCBI Gene Symbol

JAK2

Protein Name

O60674

Gene Name URL

JAK2

Nucleotide Accession Number

NM_004972.3

Frequently Asked Questions

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