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LIG1 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

DNA ligase 1

Gene Aliases

DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1.

Gene ID

81513

Accession Number

NP_001019439.1

Reactivity

Rat, Rattus norvegicus

Target

DNA ligase 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LIG1 ELISA Kit (Rat) (OKEH01916) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LIG1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LIG1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LIG1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 112%

Sensitivity

41.1 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

101 kDa

Specificity

Natural and recombinant Rat DNA ligase 1

Protein Length

913

NCBI Gene Symbol

LIG1

Protein Name

Q9JHY8

Gene Name URL

LIG1

Nucleotide Accession Number

NM_001024628.2

Frequently Asked Questions

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