LIG1 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
DNA ligase 1
Gene Aliases
DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1.
Gene ID
81513
Accession Number
NP_001019439.1
Reactivity
Rat, Rattus norvegicus
Target
DNA ligase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LIG1 ELISA Kit (Rat) (OKEH01916) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LIG1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LIG1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LIG1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 112%
Sensitivity
41.1 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
101 kDa
Specificity
Natural and recombinant Rat DNA ligase 1
Protein Length
913
NCBI Gene Symbol
LIG1
Protein Name
Q9JHY8
Gene Name URL
LIG1
Nucleotide Accession Number
NM_001024628.2
Frequently Asked Questions
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