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C19orf10 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Myeloid derived growth factor

Gene Aliases

C19orf10; EUROIMAGE1875335; IL25; IL27; IL27w; interleukin 27 working designation; interleukin-25; myeloid-derived growth factor; R33729_1; SF20; stromal cell-derived growth factor SF20; UPF0556 protein C19orf10.

Gene ID

56005

Accession Number

NP_061980.1

Reactivity

Homo sapiens, Human

Target

Myeloid-derived growth factor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology C19orf10 ELISA Kit (Human) (OKEH01901) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C19orf10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C19orf10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C19orf10 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

10 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

18 kDa

Specificity

Natural and recombinant Human UPF0556 protein C19orf10

Protein Length

173

NCBI Gene Symbol

MYDGF

Protein Name

Q969H8

Gene Name URL

MYDGF

Nucleotide Accession Number

NM_019107.3

Frequently Asked Questions

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