RUNX2 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
RUNX family transcription factor 2
Gene Aliases
Acute myeloid leukemia 3 protein; AML3; CBFA1; CBF-alpha-1; CCD; CCD1; CLCD; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; oncogene AML-3; OSF2; OSF-2; osteoblast-specific transcription factor 2; PEA2aA; PEA2-alpha A; PEBP2aA; PEBP2-alpha A; polyomavirus enhancer-binding protein 2 alpha A subunit; runt related transcription factor 2; runt-related transcription factor 2; SL3/AKV core-binding factor alpha A subunit; SL3-3 enhancer factor 1 alpha A subunit.
Gene ID
860
Accession Number
NP_001015051.3
Reactivity
Homo sapiens, Human
Target
Runt-related transcription factor 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology RUNX2 ELISA Kit (Human) (OKEH01897) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RUNX2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RUNX2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RUNX2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
Sensitivity
0.056 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
57 kDa
Specificity
Natural and recombinant Human Runt-related transcription factor 2
Protein Length
521
NCBI Gene Symbol
RUNX2
Protein Name
Q13950
Gene Name URL
RUNX2
Nucleotide Accession Number
NM_001015051.3
Frequently Asked Questions
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