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TNFRSF10A ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

TNF receptor superfamily member 10a

Gene Aliases

APO2; CD261; cytotoxic TRAIL receptor; death receptor 4; DR4; TNF-related apoptosis-inducing ligand receptor 1; TRAIL receptor 1; TRAILR1; TRAILR-1; TRAIL-R1; tumor necrosis factor receptor superfamily member 10A; tumor necrosis factor receptor superfamily member 10a variant 2; tumor necrosis factor receptor superfamily, member 10a.

Gene ID

8797

Accession Number

NP_003835.3

Reactivity

Homo sapiens, Human

Target

Tumor necrosis factor receptor superfamily member 10A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TNFRSF10A ELISA Kit (Human) (OKEH01845) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNFRSF10A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNFRSF10A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TNFRSF10A captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%

Sensitivity

0.1 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

50 kDa

Specificity

Natural and recombinant Human Tumor necrosis factor receptor superfamily member 10A

Protein Length

468

NCBI Gene Symbol

TNFRSF10A

Protein Name

O00220

Gene Name URL

TNFRSF10A

Nucleotide Accession Number

NM_003844.3

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