TNFRSF12A ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
TNF receptor superfamily member 12A
Gene Aliases
CD266; FGF-inducible 14; fibroblast growth factor-inducible immediate-early response protein 14; FN14; tumor necrosis factor receptor superfamily member 12A; TWEAKR; tweak-receptor; type I transmembrane protein Fn14.
Gene ID
51330
Accession Number
NP_057723.1
Reactivity
Homo sapiens, Human
Target
Tumor necrosis factor receptor superfamily member 12A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TNFRSF12A ELISA Kit (Human) (OKEH01840) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNFRSF12A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNFRSF12A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TNFRSF12A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
0.067 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
13 kDa
Specificity
Natural and recombinant Human Tumor necrosis factor receptor superfamily member 12A
Protein Length
129
NCBI Gene Symbol
TNFRSF12A
Protein Name
Q9NP84
Gene Name URL
TNFRSF12A
Nucleotide Accession Number
NM_016639.2
Frequently Asked Questions
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