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ZAP70 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Zeta chain of T cell receptor associated protein kinase 70

Gene Aliases

70 kDa zeta-associated protein;70 kDa zeta-chain associated protein; ADMIO2; IMD48; SRK; STCD; STD; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP-70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD.

Gene ID

7535

Accession Number

NP_001070.2

Reactivity

Homo sapiens, Human

Target

Tyrosine-protein kinase ZAP-70

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ZAP70 ELISA Kit (Human) (OKEH01831) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ZAP70 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ZAP70 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ZAP70 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%

Sensitivity

0.2 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

70 kDa

Specificity

Natural and recombinant Human Tyrosine-protein kinase ZAP-70

Protein Length

619

NCBI Gene Symbol

ZAP70

Protein Name

P43403

Gene Name URL

ZAP70

Nucleotide Accession Number

NM_001079.3

Frequently Asked Questions

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