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ADAL ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Adenosine deaminase like

Gene Aliases

Adenosine deaminase-like protein; HsMAPDA; N6-mAMP deaminase; N6-methyl-AMP aminohydrolase.

Gene ID

161823

Accession Number

NP_001012987.1

Reactivity

Homo sapiens, Human

Target

Adenosine deaminase-like protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology ADAL ELISA Kit (Human) (OKEH01830) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ADAL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ADAL and incubated. The ADAL found in the sample or standards competes with the biotinylated ADAL for limited binding sites on the immobilized anti-human ADAL antibody. Excess unbound biotinylated ADAL and sample or standard ADAL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ADAL captured in the well and inversely proportional to the amount of ADAL which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 80%

Sensitivity

0.17 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

40 kDa

Specificity

Natural and recombinant Human Adenosine deaminase-like protein

Protein Length

355

NCBI Gene Symbol

ADAL

Protein Name

Q6DHV7

Gene Name URL

ADAL

Nucleotide Accession Number

NM_001012969.3

Frequently Asked Questions

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