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AEBP1 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

AE binding protein 1

Gene Aliases

A; ACLP; adipocyte enhancer-binding protein 1; aortic carboxypeptidase-like protein.

Gene ID

11568

Accession Number

NP_001278786.1

Reactivity

Mouse, Mus musculus

Target

Adipocyte enhancer-binding protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology AEBP1 ELISA Kit (Mouse) (OKEH01823) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AEBP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AEBP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AEBP1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 80%

Sensitivity

42.1 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

128 kDa

Specificity

Natural and recombinant Mouse Adipocyte enhancer-binding protein 1

Protein Length

1128

NCBI Gene Symbol

Aebp1

Protein Name

Q640N1

Gene Name URL

Aebp1

Nucleotide Accession Number

NM_001291857.2

Frequently Asked Questions

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