AEBP1 ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
AE binding protein 1
Gene Aliases
A; ACLP; adipocyte enhancer-binding protein 1; aortic carboxypeptidase-like protein.
Gene ID
11568
Accession Number
NP_001278786.1
Reactivity
Mouse, Mus musculus
Target
Adipocyte enhancer-binding protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology AEBP1 ELISA Kit (Mouse) (OKEH01823) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AEBP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AEBP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AEBP1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 80%
Sensitivity
42.1 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
128 kDa
Specificity
Natural and recombinant Mouse Adipocyte enhancer-binding protein 1
Protein Length
1128
NCBI Gene Symbol
Aebp1
Protein Name
Q640N1
Gene Name URL
Aebp1
Nucleotide Accession Number
NM_001291857.2
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