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C9orf140 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Suppressor APC domain containing 2

Gene Aliases

2010317E24Rik; C9orf140; p42.3; protein C9orf140; suppressor APC domain-containing protein 2; TS/MDEP; tumor specificity and mitosis phase-dependent expression protein.

Gene ID

89958

Accession Number

NP_848543.2

Reactivity

Homo sapiens, Human

Target

Suppressor APC domain-containing protein 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology C9orf140 ELISA Kit (Human) (OKEH01817) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human C9orf140 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated C9orf140 and incubated. The C9orf140 found in the sample or standards competes with the biotinylated C9orf140 for limited binding sites on the immobilized anti-human C9orf140 antibody. Excess unbound biotinylated C9orf140 and sample or standard C9orf140 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated C9orf140 captured in the well and inversely proportional to the amount of C9orf140 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%

Sensitivity

0.43 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

42 kDa

Specificity

Natural and recombinant Human Suppressor APC domain-containing protein 2

Protein Length

394

NCBI Gene Symbol

SAPCD2

Protein Name

Q86UD0

Gene Name URL

SAPCD2

Nucleotide Accession Number

NM_178448.3

Frequently Asked Questions

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