C9orf140 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Suppressor APC domain containing 2
Gene Aliases
2010317E24Rik; C9orf140; p42.3; protein C9orf140; suppressor APC domain-containing protein 2; TS/MDEP; tumor specificity and mitosis phase-dependent expression protein.
Gene ID
89958
Accession Number
NP_848543.2
Reactivity
Homo sapiens, Human
Target
Suppressor APC domain-containing protein 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology C9orf140 ELISA Kit (Human) (OKEH01817) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human C9orf140 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated C9orf140 and incubated. The C9orf140 found in the sample or standards competes with the biotinylated C9orf140 for limited binding sites on the immobilized anti-human C9orf140 antibody. Excess unbound biotinylated C9orf140 and sample or standard C9orf140 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated C9orf140 captured in the well and inversely proportional to the amount of C9orf140 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%
Sensitivity
0.43 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
42 kDa
Specificity
Natural and recombinant Human Suppressor APC domain-containing protein 2
Protein Length
394
NCBI Gene Symbol
SAPCD2
Protein Name
Q86UD0
Gene Name URL
SAPCD2
Nucleotide Accession Number
NM_178448.3
Frequently Asked Questions
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